Near Infrared Spectroscopy: A useful technique for inline monitoring of the enzyme catalyzed biosynthesis of third-generation biodiesel from waste cooking oil

Near Infrared (NIR) spectroscopy was used for inline monitoring the enzymatic production of third-generation biodiesel from waste cooking oil (WCO) —with fatty acid profile similar to olive oil— under principles Continued Process Verification (CPV) and Analytical Technology (PAT). For this purpose, covalently immobilized mature Rhizopus oryzae lipase (rROL) transesterify WCO with methanol ethanol, firstly in 10 mL vials orbital stirring then it successfully scaled up a mechanically stirred 50 laboratory reactor specially designed use NIR probe. Biocatalyst half-life productivity after ten reaction cycles ethanol were assessed. Slightly higher operational stability (337.5 h, 54 batches) vs (146.7 44 observed, but decreased as result increased times former (219 327 µmol min?1). The results highly correlated those gas chromatography (GC) reference. Thus, root mean-square standard error prediction (RMSEP) 2.0% 2.1% ethanol. Therefore, spectroscopy, which allows data acquisition real time, is suitable biodiesel.